Ultrasound-mediated Non-invasive Gene Transfer Nikolitsa Nomikou，1 Anthony P. McHale，
1
Sonidel Ltd.， Dublin， Ireland and 2 School of Biomedical Sciences， University of Ulster， Coleraine， Co. Derry， BT52 1SA，超声转染， Northern Ireland
讨论和结论超声可用于在体外或体内刺激位点特异性***表达，该技术提供的非***性时空控制程度在***工程/再生***等领域具有重大潜力
Discussion and Conclusi Ultrasound can be used to stimulate site-specific gene expression either in vitro or in vivo and the
degree of non-invasive spatiotemporal control afforded by this technology offers significant potential in areas such as tissue
engineering/regenerative medicine
材料与方法分别以RIF-1细胞和C3H/HeN小鼠为体外和体内。Sonidel SP100
使用产生超声波和微泡用于增强***转移。
荧
结果初在96例患者中进行了***转移-孔板配置，这被发现与高通量应用高度兼容。为了证明超声介导的***转移的位点特异性，超声转染NEPA GENE，采用了基于Opticell?的配置，数据如下图1A所示。
Results
Initially gene transfer was performed in a 96- well te configuration and this was found to be highly compatible with high throughput application. In order to demtrate the site specific nature of ultrasound-mediated gene transfer an Opticell?-based configuration was employed and data are shown in Fig. 1A below.
The patterns of gene expression detected in tes indicate the high degree of spatialspecificity achievable with this approach. In
extrapolating the approach to demtrate site specific gene transfer and expression in vivo，
应用文章
Human Concentrative Nucleoside Transporter 3 Transfection with Ultrasound and Microbubbles in Nucleoside Transport eficient
HEK293 Cells Greatly Increases Gemcitabine Uptake. Robert J. Paproski，超声转染安全性， Sylvia Y. M. Yao， Nicole F***is， D***id Evans，聚焦超声研究（FUS， James D. Young，
Carol E. Cass， Roger J. Zemp
PLoS ONE Published: February 18， 2013
超声和微泡转染核苷转运缺陷型人浓缩核苷转运体3
HEK293细胞显著增加吉西他滨的摄取。罗伯特·J·帕普罗斯基、姚、法维斯、埃文斯、杨、，
卡罗尔·E·卡斯、罗杰·J·泽普
《公共科学图书馆一号》出版日期：2013年2月18日
仪器设备(图)-超声转染NEPA GENE-超声转染由世联博研（北京）科技有限公司提供。世联博研（北京）科技有限公司为客户提供“细胞力学设备,微观生物力学设备,生物打印机,电子材料打印机”等业务，公司拥有“世联博研”等品牌，专注于科研仪器仪表等行业。，在北京市昌平区回龙观镇上奥世纪中心2B座6层603的名声不错。欢迎来电垂询，联系人：李经理。