应用领域
- High-sensititivity detection of RNA, including RNA present at low copy number.
- 高灵敏度检测RNA，包括低拷贝数的RNA。
- RT-PCR applications where sensitivity is the primary concern.
- RT-PCR应用中，灵敏度是最主要的问题。
- Multiplex RT-PCR.
- 多重RT-PCR。
用于高灵敏度的MasterAmp™RT-PCR试剂盒是一种单酶，单管RT-PCR系统（图1）。它能够从低至1 pg的细胞总RNA中扩增RNA（图2）。它是扩增低拷贝数RNA转录本的理想选择。
The MasterAmp™ RT-PCR Kit for High Sensitivity is a single enzyme, single-tube RT-PCR system (Fig. 1). It is capable of amplifying RNA from as little as 1 pg of total cellular RNA (Fig. 2). It is ideal for amplifying low-copy-number RNA transcripts.
用于高灵敏度的MasterAmp RT-PCR试剂盒使用RetroAmp™RT DNA聚合酶，这是一种热稳定酶，在≥60°C时具有峰值逆转录酶活性，而没有RNase H活性。较高温度下的逆转录减少RNA二级结构，并增加cDNA合成的特异性和敏感性。由于RT和PCR均发生在单个试管中，因此该试剂盒的灵敏度进一步提高。RT期间合成的所有cDNA均用作PCR扩增的模板。
The MasterAmp RT-PCR Kit for High Sensitivity uses RetroAmp™ RT DNA Polymerase, a thermostable enzyme that has peak reverse transcriptase activity at ≥60°C and lacks RNase H activity. Reverse transcription at higher temperatures reduces RNA secondary structure and increases the specificity and sensitivity of cDNA synthesis. The sensitivity of this kit is further augmented because both RT and PCR occur in a single tube. All cDNA synthesized during RT is used as a template for PCR amplification.
该试剂盒还整合了具有甜菜碱的MasterAmp PCR增强剂*，可通过减少DNA合成过程中的停顿和终止来进一步提高RT-PCR的特异性，一致性和敏感性。
The kit also incorporates MasterAmp PCR Enhancer with betaine,* which further improves the specificity, consistency, and sensitivity of RT-PCR by reducing pauses and stops during DNA synthesis.
注意：尽管用于高灵敏度的MasterAmp RT-PCR试剂盒提供了无与伦比的RT-PCR灵敏度，但当高保真度最高时，应使用MasterAmp高保真RT-PCR试剂盒通过RT-PCR扩增RNA进行克隆，测序或表达。重要。
Note: Although the MasterAmp RT-PCR Kit for High Sensitivity provides unsurpassed RT-PCR sensitivity, the MasterAmp High Fidelity RT-PCR Kit should be used to amplify RNA by RT-PCR for cloning, sequencing, or expression, when high fidelity is most important.
优势说明
- 少至1 pg的细胞总RNA即可进行高灵敏度RT-PCR（图2）。
- 优于其他供应商的同类产品。
- High-sensitivity RT-PCR from as little as 1 pg of total cellular RNA (Fig. 2).
- Outperforms similar products from other vendors.
- Thermostable RetroAmp™ RT DNA Polymerase enables RT to be performed at higher temperature, which minimizes RNA secondary structure.
- Use of one enzyme in a one-step and one-tube reaction with no sample transfers reduces the chance for cross-contamination.
- The MasterAmp PCR Enhancer technology increases RT-PCR sensitivity and specificity.
- Thermostable RetroAmp™RT DNA聚合酶可使RT在更高的温度下进行，从而最大程度地减少了RNA二级结构。
- 一种酶的一步和一管式反应，无需样品转移，可减少交叉污染的机会。
- MasterAmp PCR Enhancer技术可提高RT-PCR的灵敏度和特异性。
epicentre RT71225 MasterAmp™ RT-PCR Kit for High Sensitivity 图例说明
