十二烯基丁二酸酐DDSA
Dodecenylsuccinic Anhydride
Wako Pure Chemicals
Grade: for Electron Microscopy
Regulation / Hazardous:
Storage condition: Protect from light.
Manufacturer: Wako Pure Chemicals
Summary
<Histochemistry and Cytochemistry><Embedding Reagent>
Embedding give c***istent and uniform hardness to tissues (blocks) as well as strength not deforming during sectioning by filling the hollow parts within the tissues. It also increases preservability including easiness for handling.
While embedding with paraffin is usually employed for light microscopic observation and that with various resins for electron microscopic observation, use of freeze-sectioning instead of embedding is increasing.
<For electron microscope>
Various resins including epoxy resins are used as embedding agents for electron microscopy to meet the following requirements.
- Not contracting upon polymerization.
- Monomers are easy to handle and penetrate into tissues.
- Clear and preferably colorless.
- Polymerized resins are resistant to electron beams.
- Polymers of appropriate hardness can be prepared.
- Easy to section.
Epoxy resin embedding materials are adjustable for hardness by altering the type and amount of addition of a hardening agent, polymerization catalyst, accelerator, etc.
<Hardening agent>
Molecular Weight 266.38
Purity: 95.0+% (Titration)
Melting Point: < 0 degrees C
Flash Point: 185 degrees C
Density: 1.00 - 1.02g/ml (20 degrees C)
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