肽链内切酶Lysyl Endopeptidase(R)
规格: 1 vial (2 AU) , 1 vial (10 AU)
Grade: for Biochemistry
Summary
This product is for research use only. Do not administer it to human.
Purity: Homogenous on SDS-PAGE
Activity: shown on each label. Unit: AU*/vial
* AU: Amidase Unit - amount of enzyme required for the formation of 1
umol of p-nitroaniline from
N-benzoyl-DL-lysine-p-nitroaniline per minute at 30 degrees C, pH 9.5.
Preparation A 10AU-vial contains Tris-HCl corresponding to 1 mL of 2
mM Tris HCl buffer (pH 8.0).
Molecular Weight] 27,000 (gel filtration); 30,000 (SDS
electrophoresis)
Optimal pH 9.0 -9.5 (amidase activity)
Stability Very stable in lyophilized forms at -20 degrees C; Stable
for at least 2 years at -20 degrees C
when dissolved in Tris-HCl buffer at pH 4.0 to 11 at the enzyme
concentration of more than 1 mg/mL. Stable for at
least 24 hours at 4 degrees C under the above conditi***.
Lysyl Endopeptidase is a protease secreted by the soil bacterium
Achromobacter lyticus M497-1,
and is synonymous with the achromobacter protease discovered by
Masaki, et al. Lysyl Endopeptidase
cle***es peptide bonds at the carboxy-terminal of both lysine residues
and S-aminoethylcysteine residues with a
high degree of specificity, making it a valuable tool for analysis of
peptide and protein primary structure and for
enzymatic synthesis of Lys-X compounds. An added feature of Lysyl
Endopeptidase is its ability to retain complete
activity after incubation in 4M urea or in 0.1% SDS solution for up to
6 hours at 30 degrees C.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~
如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com 或
在我公司查询主页客户留言处（http://www.express-
cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复
。
伊普瑞斯科技有限公司
info@express-cn.com
总机：010-60206266
传真：010-60206773
www.express-cn.com