l Before use, mix all reagents thoroughly without making foam.
l Determine the number of microwell strips required to test the desired number of samples,plus appropriate number of wells needed for running blanks standa***. Each sample, standard and blank should be assayed in duplicate. Remove sufficient microwell strips from the pouch.
l Add 50ul of standard diluent to standard wells B1,B2, C1,C2, D1,D2, E1,E2, F1,F2. Rec***titute standard vial with the appropriate volume as described in the chapter reagents preparation. Preparation. Pipet 100ul of standard into wells A1 and A2 (see plate scheme below). Transfer 50ul from A1 and A2 to B1 and B2 wells. Mix the contents by repeated aspirati*** and ejecti***. Take care not to scratch the inner surface of microwells. Repat this procedure from the wells B1,B2 to wells C1,C2 and from wells C1,C2 to D1,D2 and so on creating two parallel rows of porcine insulin standard diluti*** ranging，Add 50ul of standard diluent to the bland wells.
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