PREPARATION OF REAGENTS
l Standa***: Standard h***e to be rec***tituled with the volume of standard buffer diluent indicated on the vial. This rec***titution produces a stock solution of 80uIU/ml porcine insulin. Allow standard to stand for 5
l minutes with gentle swirling prior to making diluti***. Serial diluti*** of standard must be made before each assys and cannot be stored.
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80 uIU/ml
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（6 Standard）
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Original density 50ul。
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40 uIU/ml
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（5 Standard）
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100ul 6 Standard +100ul diludent
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20 uIU/ml
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（4 Standard）
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100ul 5 Standard +100ul diludent
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10 uIU/ml
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（3 Standard）
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100ul 4 Standard +100ul diludent
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5.0uIU/ml
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（2 Standard）
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100ul 3 Standard +100ul diludent
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2.5 uIU/ml
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（1 Standard）
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100ul 2 Standard +100ul diludent
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0 uIU/ml
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Blank Control
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50ul。
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