SPECIMEN COLLECTION\ PROCESSING AND STORAGE
l Serum---***oid any inintentional stimulation of the cells by the procedure. Use pyrogen\endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000×g for 10 min and remove serum.
l Pla***a---EDTA\ citrate and heparin pla***a can be assayed. Spin samples at 1000×g for 30 min remove particulates. Harvest pla***a.
l Cell culture supernatants---Remove particulates and aggregates by spinning at approximately 1000×g for 10 min.
l Storage---If not analyzed shortly after collection, samples should be aliquoted(250-500ul) to ***oid freeze-thaw cycles and stored frozen at -70℃. ***oid multiple freeze-thaw cycles of frozen specimens. When possible, ***oid use of badly hemolyzed or lipemic sera. If large amounts of particles are present, this should be removed prior to assay by centrifugation or filtration.
Recommendation---Do not thaw by heating at 37℃ or 56℃. Thaw at room temperature and make sure that sample is completely thawed and homogenous befo说明书Email:info@索取;13801829628定货； HR-M0414