microRNA Cloning Kit wako
Grade: for Genetic Research
Regulation / Hazardous: UN: Class 8 - Corrosive
Storage condition: Keep at -20 degrees C.
Cat. No. 290-66501
Size : 8Tests
Summary
This product is for research use only. Do not administer it to human.
The microRNA Cloning Kit Wako can prepare cDNA encoding microRNA is completed within 1.5 days after extraction of ***all RNA fraction by using denatured polyacrylamide gel and spin column extraction.
The existing methods of microRNA cloning h***e following some problems, several extraction by denatured polyacrylamide gel, many steps of RNA handling, use of RI, use of expensive apparatus such as an imaging analyzer.
The microRNA Cloning Kit Wako is supported by shrimp alkaline phosphatase (SAP), thermostable single strand DNA ligase (selling separately), and original modified adaptors.
The cloning efficiency using this kit is improved higher than that of the conventional methods, which used bacterial alkaline phosphatase and T4 RNA ligase.
Note:
This kit must be used together with Single Strand DNA Ligase, Thermostable (Wako Cat.#298-65103, 200 units), which is sold separately as a stand-alone product. This ligase will be used for ligation of ssDNA-ssDNA, ssRNA-ssRNA and ssDNA-ssRNA depending on ATP. Reaction of this ligase in 55-65 degrees, which is the optimum reaction temperature, will promote the ligation efficiency.
Features
High cloning efficiency
Cloning of secondary structured microRNA
High reproducibility of microRNA cloning
Kit Contents (for 8 reacti***)
SAP: 1 vial x 16 uL
5x SAP Buffer: 1 vial x 64 uL
40x Ligation Buffer: 1 vial x 16 uL
RNase Inhibitor: 1 vial x 16 uL
10 mmol/L MnCl2: 1 vial x 16 uL
Reverse Transcriptase: 1 vial x 8 uL
10x RT Buffer: 1 vial x 16 uL
dNTP Mixture: 1 vial x 112 uL
0.5 mol/L EDTA, pH 8.0: 1 vial x 16 uL
1 mol/L Tris-HCl, pH 7.5: 1 vial x 160 uL
Ethachinmate: 1 vial x 24 uL
10 mol/L Ammonium Acetate: 1 vial x 960 uL
3' Adaptor (50 pmol/uL): 1 vial x 8 uL
5' Adaptor (50 pmol/uL): 1 vial x 8 uL
RT Primer (50 pmol/uL): 1 vial x 8 uL
5' PCR Primer (50 pmol/uL): 1 vial x 16 uL
3' PCR Primer (50 pmol/uL): 1 vial x 16 uL
Control RNA (30 ng/uL): 1 vial x 8 uL
microRNA Cloning Kit Wako is patent pending. (1, 10, 2007)
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uL: microlitters
说明书单询