LabAssay (TM) Triglyceride (甘油三酸酯***盒)
Grade: for Cellbiology
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd.
Cat. No. 290-63701
Pkg. Size 1000Tests
Summary
This product is for research use only. Do not administer it to human.
Lipids in serum c***ist of triglycerides, cholesterols, phospholipids, free fatty acids and slight amounts of fat-soluble components such as fatsoluble vitamins and carotenes. Triglycerides, as major components of very low density lipoprotein (VLDL) and chylomicr***, play an important role in metaboli*** as energy sources and transporters of dietary fat.
LabAssay(TM) Triglyceride is based on an enzymatic method using N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS)
as a blue pigment. This kit is used for the quantitative determination of triglycerides in mouse serum. It is a simultaneous multi-sample assay format using a microplate, but measurements can also be made using a test tube.
[Kit Contents]
Buffer (50 mmol/L Good's (PIPES) buffer, pH 6.5) 3 vials x
105 mL
Chromogen Substrate 3 vials x for 105 mL
(when rec***tituted)
Lipoprotein lipase 99 units/mL
Adenosine 5'-Triphosphate Disodium Salt Trihydrate (ATP) 1.7 mmol/L
Glycerolkinase 30 units/mL
Glycerol-3-phosphate Oxidase (GPO) 1.0 unit/mL
Peroxidase (POD) 5.5 units/mL
N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) 0.54 mmol/L
4-Aminoantipyrine 0.11 mmol/L
Ascorbate Oxidase 4.5 units/mL
(before rec***titute)
Contains Sodium Hydroxide 8.01%.
Standard Solution 1 vial x 10 mL
Glycerol 31.2 mg/dL
(Corresponding to 300 mg/dL Triolein)
[Assay principle]
Triglycerides in a sample are hydrolyzed to glycerol and free fatty
acids in a reaction catalyzed by lipoprotein lipase (LPL). Glycerol is converted to glycerol-3-phosphate by glycerolkinase (GK) in the presence of ATP.
Glycerol-3-phosphate so formed is oxidized by glycerol-3-phosphate oxidase (GPO) in a reaction that produces hydrogen peroxide. The hydrogen peroxide produced causes DAOS and 4-Aminoantipyrine to undergo a quantitative oxidative condensation catalysed by peroxidase (POD), producing a blue pigment. The amount of triglycerides contained in the sample is determined by measuring the absorbance of the blue color.
[Performance]
Sensitivity (assay in a test tube)
The absorbance is below 0.10, when measuring purified water as a sample.
The absorbance is 0.09 to 0.25, when measuring 300 mg/dL triglycerides as a sample.
Specificity
The triglycerides concentration is less than &plu***n;12%, when measuring
the known concentration of control serum as a sample.
[Expiration date] 12 months after
the manufacture
[Reference] Spayd, R. W.,
Bruschi, B. et al.: Clin. Chem., 24, 1343 (1978).