S. cerevisiae Direct Transformation Kit Wako(酵母S.Cerevisiae直接转化***盒)
Grade: for Genetic Research
Storage condition: Keep at -20 degrees C.
Cat. No. 296-62701 20tests
Cat. No. 292-62703 100tests
Cat. No. 290-62704 500tests
Summary
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This product is for research use only. Do not administer it to human.
for genome-wide screening!!
The S. cerevisiae Direct Transformation Kit Wako is specially designed for easy transformation of the budding yeast, Saccharomyces sp. The S. cerevisiae Direct Transformation Protocol allows successful transformation simply by mixing a pla***id DNA and the kit reagents with cultured yeast cells. No complicated steps, such as centrifugation or cell washing, are required. The S. cerevisiae Direct Transformation Kit Wako is particularly well suited for high-throughput transformationof a large number of yeast strains grown in 96-well plates. Even genome-wide screening, which has been problematic, becomes possible, since the S. cerevisiae Direct Transformation Kit Wako easily transforms about 4,850 strains with gene disruption.
Features
No centrifugation or washing steps are required.
One-step procedure by mixing pla***id DNA and the kit reagents with cultured yeast cells.
Suitable for transformation of a large number of yeast strains using 96-well plates.
Applicable to tube protocol.
Low-viscosity reagent enables high-throughput screening.
Transformation Efficiency BY4743 strain with pRS316 pla***id
96-well plate protocol: 500 and over
Tube protocol: 5,000 and over
The product information is shown on the page #10 of Wako Product Update No.15.