酪胺酸酶Tyrosinase from mushroom
BioChemika, lyophilized, ≥2000 units/mg
Descripti***
Other Notes Kinetic study of the *** inactivation of tyrosinase1 ; Chromogenic assay
Unit Definition 1 U corresponds to the amount of enzyme which increases the absorbance at 305 nm by 0.001 per minute at pH 6.5 and 25°C (L-tyrosine as substrate, 3.0 mL reaction mix); 30 absorbance-U as described above are equivalent to ~1 U [when 1 U is the amount of enzyme which oxidyzes 1 μmol 4-methylcatechol per minute at pH 6.5 and 25°C]
General description Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is resp***ible for browning reacti***. The enzyme is reported to h***e two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Properties
mol wt
Mr ~125000
solubility
0.02 M phosphate, pH 6.5: 5mg/mL, clear, slightly brown to deep brown
storage temp.
?20°C
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com 或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。
伊普瑞斯科技有限公司
info@express-cn.com
在线***：***：1069267096
MSN:liyuqiu1983@
cindylee-1981@
总机：010-60206266
传真：010-60206773
www.express-cn.com