Rat C-Peptide ELISA Kit （大鼠C肽Elisa***盒）
Grade: for Diabetes Research
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd.
Cat. No. Pkg. Size
295-57401 96Tests
Summary
This product is for research use only. Do not administer it to human.
[Kit Contents]
Antibody coated Microtiter Plate: 1 plate
Rat C-Peptide Standard: 50 ng
Biotinylated Rat C-Peptide: for 8 mL
Anti Rat C-Peptide, Rabbit: 12 mL
HRP-conjugated Streptzvidin: 12 mL
Chromogen (OPD Tablet): 2 tablets
Wash Stock Solution (20 x): 50 mL
Buffer: 35 mL
Stop Solution (1 mol/L H2SO4): 12 mL
Adhesive Plate Cover: 3 covers
[Principe]
On the surface of the microplate wells, goat anti rabbit IgG is coated and when sample is reacted with rabbit and InsC-Peptide and biotinylated rat InsC-peptide in the well, the goat antibody captures the complex of the rabbit antibody and rat Ins C-peptide formed in competitive reaction.
Subsequentreaction with horseradish peroxides (HRP)-conjugated Streptzvidin to the complex on the well surface results in labeling of the complex with HRP which generates the signal for the presence of rat InsC-peptide in the sample.
[Capability]
Dynamic range: 0.56 - 50 ng/mL
Cross-reactivity: Specific to rat InsC-Peptide, but not reactive to either the cooresponding of the other animal.
Recovery data (serum): 101-107 %
说明书单询。
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