氨基反应的生物素交联剂，NHS-Biotin，NHS-LC-LC-Biotin，Sulfo NHS-Biotin
西安瑞禧生物科技有限公司提供与氨基反应的生物素交联剂，介绍如下：
N-hydroxysuccinimidyl esters (NHS) of biotins are excellent reagents for biotinylating biomolecules such as proteins and amine-modified nucleic acids. NHS-Biotin (Order-No. B001N) is the simplest biotinylating reagent ***ailable. When the target amines are less reactive (i.e. secondary amines) a fluorophenol active ester like PFP-Biotin (Order-No. B010F) is preferred. Both reagents are insoluble in aqueous media, and must be first dissolved in non-protic organic solvents such as *** or DMSO, then aliquot into the aqueous reaction mixture. Because these compounds are prone to hydrolysis by water, it is recommended to prepare fresh stock soluti*** to achieve the best yield at a final concentration of organic solvent not above 10%. Similar procedures are employed for:
NHS-LC-Biotin
NHS-LC-LC-Biotin
Water-soluble analogs of NHS ester biotins are also ***ailable, like:
Sulfo NHS-Biotin (Order-No. B101N)
Sulfo NHS-LC-Biotin (Order-No. B102N)
Sulfo NHS-LC-LC-Biotin (Order-No. B103N)
These active esters contain a negatively charged sulfonate in their NHS ring structure. They can be added directly to aqueous reaction media, without the need of organic solvents. Concentrated stock soluti*** may be prepared, but must used immediately as they hydrolyze quickly. Another water-soluble Sulfo NHS ester like Sulfo NHS-SS-Biotin (Order-No. B104N) contains a spacer arm which can be cle***ed under mild conditi***. Reduction of the disulfide bond with 50 mM dithiothreitol releases the biotinylated component from the ***idine or strept***idine labeled detection reagents. Cyanagen developed a new class of water-soluble Biotin NHS esters based on hydrophilic polyethylene oxide (PEO) spacer arms like:
NHS-PEO3-Biotin (Order-No. B040N)
NHS-PEO4-Biotin (Order-No. B041N)
NHS-PEO5-Biotin (Order-No. B042N)
详细的生物素交联***的产品信息如下：
NHS-Biotin MW: 341.38
NHS-LC-Biotin MW: 454.54
NHS-LC-LC-Biotin MW: 567.70
NHS-PEO3-Biotin MW: 500.58
NHS-PEO4-Biotin MW: 544.63
NHS-PEO5-Biotin MW: 588.68
Sulfo NHS-Biotin MW: 443.43
Sulfo NHS-LC-Biotin MW：556.60
Sulfo NHS-LC-LC-Biotin MW：669.74
Sulfo NHS-SS-Biotin MW：606.70
PFP-Biotin MW：410.36
订购联系信息：
西安瑞禧生物科技有限公司
电话：029-86354885 13309186942 ***:31952518
e-mail:sales@