荧光染料激发波长、发射波长
以下数据仅供参考，根据厂商提供的染料光谱特性可能有所变化，建议您提供厂家的染料光谱及激发、发射特性光谱信息，以便瑞研光学帮您选择合适配套的荧光滤光片。
|
荧光染料 |
激发波长 |
发射波长 |
|
Alexa Fluor® 350 |
342 |
442 |
|
Alexa Fluor® 488 |
499 |
520 |
|
Alexa Fluor® 514 |
518 |
543 |
|
Alexa Fluor® 532 |
531 |
554 |
|
Alexa Fluor® 546 |
556 |
572 |
|
Alexa Fluor® 555 |
553 |
565 |
|
Alexa Fluor® 568 |
579 |
603 |
|
Alexa Fluor® 594 |
590 |
618 |
|
Alexa Fluor® 633 |
631 |
647 |
|
Alexa Fluor® 647 |
653 |
668 |
|
Alexa Fluor® 680 |
679 |
702 |
|
Alexa Fluor® 700 |
696 |
719 |
以上数据仅供参考
|
荧光染料 |
激发波长 |
发射波长 |
|
Alexa Fluor® 750 |
751 |
776 |
|
AmCyan |
458 |
489 |
|
APC (Allophycocyanin) |
650 |
660 |
|
AsRed 2 |
576 |
592 |
|
ATTO 390 |
392 |
477 |
|
ATTO 425 |
436 |
484 |
|
ATTO 465 |
453 |
507 |
|
ATTO 488 |
500 |
525 |
|
ATTO 550 |
553 |
576 |
|
ATTO 633 |
629 |
658 |
|
ATTO 647N |
645 |
668 |
|
ATTO 680 |
680 |
700 |
|
BCECF (pH 5.2) |
482 |
520 |
|
BCECF (pH 9.0) |
502 |
528 |
|
Biosearch Blue |
352 |
447 |
|
BODIPY FL/pH7.2 |
505 |
512 |
|
Brilliant Violet™ 421 |
408 |
422 |
|
Brilliant Violet™ 480 |
437 |
478 |
|
CAL Fluor® Gold 540 |
522 |
544 |
|
CAL Fluor® Orange 560 |
538 |
559 |
|
CAL Fluor® Red 590 |
569 |
591 |
|
CAL Fluor® Red 610 |
590 |
610 |
|
CAL Fluor® Red 635 |
618 |
637 |
|
Calcein |
494 |
517 |
|
Calcium Green™-1 |
507 |
529 |
|
Cerulean |
434 |
473 |
|
CFP |
433 |
475 |
|
Citrine |
514 |
529 |
以上数据仅供参考
|
荧光染料 |
激发波长 |
发射波长 |
|
Coumarin |
360 |
448 |
|
Cy2™ |
492 |
507 |
|
Cy3.5™ |
578 |
591 |
|
Cy3™ |
554 |
566 |
|
Cy5.5™ |
675 |
695 |
|
Cy5™ |
649 |
666 |
|
Cy7™ |
757 |
781 |
|
DAPI |
359 |
461 |
|
Di-8-ANEPPS non-ratiometric |
466 |
630 |
|
DiA |
457 |
586 |
|
DiD |
648 |
670 |
|
DiI |
551 |
569 |
|
DiO |
490 |
506 |
|
DiR |
750 |
782 |
|
Draq5 |
647 |
683 |
|
DsRed |
559 |
583 |
|
DyLight 350 |
351 |
434 |
|
DyLight 405 |
398 |
420 |
|
DyLight 549 |
553 |
569 |
|
DyLight 594 |
593 |
618 |
|
DyLight 633 |
620 |
649 |
|
DyLight 649 |
651 |
673 |
|
DyLight 680 |
676 |
705 |
|
DyLight 750 |
751 |
778 |
|
DyLight 800 |
770 |
795 |
|
EBFP2 |
385 |
448 |
|
ECFP |
434 |
477 |
|
EGFP |
488 |
507 |
|
Emerald GFP |
489 |
510 |
|
Eosin |
525 |
546 |
|
Ethidium bromide |
518 |
603 |
|
Ethidium homidimer-1/DNA |
527 |
617 |
|
EYFP/pH 7 |
514 |
527 |
|
FAM |
492 |
518 |
|
FITC |
495 |
519 |
|
FlAsH-***FCC |
511 |
530 |
|
Fluo-3 |
506 |
527 |
|
Fluo-4 |
494 |
516 |
|
FM™ 1-43 |
473 |
579 |
|
FM™ 4-64 |
508 |
730 |
以上数据仅供参考
|
荧光染料 |
激发波长 |
发射波长 |
|
Fura-2/Ca2+ - free |
366 |
515 |
|
Fura-2/Ca2+ - saturated |
336 |
505 |
|
FusionRed |
577 |
604 |
|
GFP |
489 |
511 |
|
HEX,SE |
533 |
559 |
|
Hoechst 33258 |
352 |
455 |
|
Indo-1/Ca2+ -free |
346 |
480 |
|
Indo-1/Ca2+ -saturated |
330 |
400 |
|
JOE |
520 |
548 |
|
Killer Red |
585 |
611 |
|
Li-Cor IRDye® 680LT |
674 |
693 |
|
Li-Cor IRDye® 800CW |
778 |
794 |
|
Lucifer yellow |
428 |
544 |
|
LysoTracker Blue |
373 |
422 |
|
LysoTracker Green |
501 |
509 |
|
LysoTracker Red |
573 |
592 |
|
LysoTracker Yellow HCK-123 |
488 |
565 |
|
mCherry |
587 |
610 |
|
mCitrine |
514 |
529 |
|
MitoTracker™ Deep Red |
641 |
662 |
|
MitoTracker™ Green |
490 |
512 |
|
MitoTracker™ Orange |
551 |
575 |
|
MitoTracker™ Red |
578 |
598 |
|
mKate2 |
588 |
633 |
|
mKO |
549 |
559 |
|
mOrange2 |
551 |
565 |
|
mPlum |
589 |
649 |
|
mRFP1 |
590 |
612 |
|
mTFP1 |
462 |
492 |
|
mWasabi |
493 |
509 |
|
NBD-X (MeOH) |
467 |
538 |
|
Nile blue |
631 |
660 |
|
NirFP |
604 |
669 |
|
Oregon Green™ 488 |
498 |
526 |
|
Oregon Green™ 514 |
513 |
532 |
|
Pacific Blue™ |
404 |
455 |
|
Propidium Iodide (PI) |
305 |
617 |
|
Pulsar™ 650 |
460 |
650 |
|
Qdot® 525 Nanocrystals |
300 |
525 |
|
Qdot® 545 Nanocrystals |
300 |
543 |
|
Qdot® 565 Nanocrystals |
300 |
564 |
|
Qdot® 585 Nanocrystals |
300 |
588 |
以上数据仅供参考
|
荧光染料 |
激发波长 |
发射波长 |
|
Qdot® 605 Nanocrystals |
300 |
602 |
|
Qdot® 625 Nanocrystals |
300 |
621 |
|
Qdot® 655 Nanocrystals |
300 |
654 |
|
Qdot® 705 Nanocrystals |
300 |
702 |
|
Qdot® 800 Nanocrystals |
300 |
792 |
|
Quasar® 570 |
548 |
566 |
|
Quasar® 670 |
647 |
670 |
|
Quasar® 705 |
690 |
750 |
|
ReAsH-***GCC |
592 |
606 |
|
Resorufin |
571 |
584 |
|
Rhod-2 |
553 |
577 |
|
Rhodamine 123 |
507 |
529 |
|
Rhodamine 6G |
525 |
547 |
|
Rhodamine Red-X |
573 |
591 |
|
ROX |
578 |
604 |
|
R-phycoerythrin |
565 |
578 |
|
SBFI/Na+ -free |
340 |
560 |
|
SBFI/Na+ -saturated |
337 |
527 |
|
Sulforhodamine 101-EtOH |
578 |
593 |
|
SYBR® Green I |
498 |
522 |
|
SYTO 60 |
650 |
681 |
|
SYTO 9 |
483 |
500 |
|
TagBFP |
402 |
457 |
|
TagRFP |
556 |
584 |
|
TAMRA |
555 |
580 |
|
tdTomato |
554 |
581 |
|
TET |
521 |
542 |
|
Tetracycline |
405 |
550 |
|
Tetramethylrhodamine dextran |
554 |
582 |
|
Texas Red® |
592 |
614 |
|
TO-PRO™-3 |
642 |
657 |
|
TRITC |
552 |
578 |
|
TurboFP650 |
592 |
650 |
|
wtGFP |
474 |
509 |
|
X-Rhod-1 Indicator |
580 |
601 |
|
ZsGreen1 |
493 |
505 |
|
ZsYellow1 |
529 |
539 |
以上数据仅供参考
|
荧光染料 |
激发波长 |
发射波长 |
|
1, 5 IAEDANS |
336nm |
482nm |
|
4-Methylumbelliferone |
360nm |
449nm |
|
5-Carboxyfluorescein (5-FAM)® |
492nm |
518nm |
|
5-Carboxynaphthofluorescein® |
598nm |
668nm |
|
5-Carboxytetramethylrhodamine(5-TAMRA)® |
542nm |
568nm |
|
5-FAM (5-Carboxyfluorescein)® |
492nm |
518nm |
|
5-ROX (carboxy-X-rhodamine) |
574nm |
602nm |
|
5-TAMRA (5-Carboxytetramethylrhodamine) |
542nm |
568nm |
|
6-Carboxyrhodamine 6G (6-CR-6G)® |
518nm |
543nm |
|
6-CR 6G® |
518nm |
543nm |
|
6-JOE |
525nm |
555nm |
|
7-Amino-4-methylcoumarin® |
351nm |
430nm |
|
7-Aminoactinomycin D (7-AAD)® |
546nm |
647nm |
|
7-Hydroxy-4-methylcoumarin (pH 9) |
360nm |
449nm |
|
AcGFP |
475nm |
505nm |
|
Acridine® orange(+DNA) |
500nm |
526nm |
|
Acridine® orange(+RNA) |
460nm |
650nm |
|
Alexa Fluor® 350 |
346nm |
442nm |
|
Alexa Fluor® 405 |
401nm |
422nm |
|
Alexa Fluor® 488 |
498nm |
520nm |
|
Alexa Fluor® 514 |
517nm |
539nm |
|
Alexa Fluor® 532 |
532nm |
553nm |
|
Alexa Fluor® 546 |
556nm |
573nm |
|
Alexa Fluor® 555 |
553nm |
568nm |
|
Alexa Fluor® 568 #4433c0 #5544cc |
578nm |
603nm |
|
Alexa Fluor® 594 |
590nm |
617nm |
|
Alexa Fluor® 633 |
632nm |
647nm |
|
Alexa Fluor® 647 |
649nm |
666nm |
|
Alexa Fluor® 680 |
680nm |
702nm |
|
Alexa Fluor® 700 |
696nm |
719nm |
|
Alexa Fluor® 750 |
752nm |
779nm |
|
Alexa Fluor® 790 |
782nm |
805nm |
|
Alexa Fluor® 488/546 FRET |
495nm |
573nm |
以上数据仅供参考
|
荧光染料 |
激发波长 |
发射波长 |
|
Alexa Fluor® 488/555 FRET |
495nm |
568nm |
|
Alexa Fluor® 488/Cy3® FRET |
495nm |
570nm |
|
Allophycocyanin (APC) |
650nm |
660nm |
|
AMCA/AMCA-X |
345nm |
445nm |
|
AmCyan |
458nm |
489nm |
|
Aniline Bluer® |
370nm |
509nm |
|
ANSr® |
372nm |
455nm |
|
AsRed2 |
576nm |
592nm |
|
Atto 390 |
390nm |
479nm |
|
Atto 425 |
436nm |
485nm |
|
Atto 465 |
453nm |
507nm |
|
Atto 488 |
501nm |
525nm |
|
Atto 550 |
554nm |
576nm |
|
Atto 633 |
630nm |
657nm |
|
Atto 647N |
644nm |
669nm |
|
Atto 680 |
680nm |
700nm |
|
ATTO-TAG™ CBQCA |
465nm |
560nm |
|
ATTO-TAr™ FQ |
486nm |
591nm |
|
Auraminer O-Feulgen |
460nm |
550nm |
|
Azamir Green |
492nm |
505nm |
|
BCECF |
503nm |
528nm |
|
BCECF/pH 5.2 |
482nm |
520nm |
|
BCECF/pH 9.0 |
503nm |
528nm |
|
BFP (Blue Fluorescent Protein)r |
382nm |
448nm |
|
BFP/DsRed2 FRET |
382nm |
583nm |
|
BFP/eGFP FRET |
382nm |
508nm |
|
BFP/YFP FRET |
382nm |
527nm |
|
Biosearch Blue |
352nm |
447nm |
|
***™-1 BO-PRO™-1 |
462nm |
481nm |
|
***™-3 BO-PRO™-3 |
570nm |
604nm |
|
BODIPY® FL/pH7.2 |
505nm |
512nm |
|
BODIPY® 530/550 |
533nm |
550nm |
|
BODIPY® 558/568 |
558nm |
568nm |
|
BODIPY® 564/570 |
564nm |
570nm |
|
BODIPY® 581/591 |
582nm |
590nm |
|
BODIPY® 630/650-X |
630nm |
650nm |
|
荧光染料 |
激发波长 |
发射波长 |
|
BODIPY® 650/665-X |
650nm |
665nm |
|
BODIPY® FL |
505nm |
513nm |
|
BODIPY® TMR |
542nm |
574nm |
|
BODIPY® TR-X |
589nm |
617nm |
|
BTC |
401nm |
529nm |
|
CAL Fluor® Gold 540 |
522nm |
544nm |
|
CAL Fluor® Orange 560 |
538nm |
559nm |
|
CAL Fluor® Red 590 |
569nm |
591nm |
|
CAL Fluor® Red 610 |
590nm |
610nm |
|
CAL Fluor® Red 635 |
618nm |
637nm |
|
Calcein |
494nm |
517nm |
|
Calcein Blue |
375nm |
420nm |
|
Calcein® |
494nm |
517nm |
|
Calcium Crimson™ |
590nm |
615nm |
|
Calcium Green-1™ |
506nm |
531nm |
|
Calcium Orange™ |
549nm |
576nm |
|
Calcofluor® White |
350nm |
440nm |
|
Carboxy-X-rhodamine (5-ROX) |
574nm |
602nm |
|
Cascade Blue® |
400nm |
420nm |
|
Cascade Yellow™ |
402nm |
545nm |
|
CCF2 (GeneBLAze™r) |
402nm |
520nm |
|
Cerulean |
433nm |
475nm |
|
CFP(Cyan Fluorescent Protein)? |
434nm |
477nm |
|
CFP/DsRed2 FRET |
434nm |
583nm |
|
CFP/YFP FRET |
434nm |
527nm |
|
Chromomycin A3 |
450nm |
470nm |
|
Citrine |
514nm |
529nm |
|
Cl-NERF (low pH) |
504nm |
540nm |
|
Coumarin |
384nm |
470nm |
|
C-phycocyanin |
620nm |
648nm |
|
*** |
385nm |
471nm |
|
CryptoLight CF-2 |
584nm |
657nm |
|
荧光染料 |
激发波长 |
发射波长 |
|
CryptoLight CF-5 |
566nm |
597nm |
|
CryptoLight CF-6 |
566nm |
615nm |
|
CTC Formazan |
450nm |
630nm |
|
Cy2™ |
489nm |
506nm |
|
Cy3.5™ |
581nm |
598nm |
|
Cy3™ /Cy5.5™ FRET |
550nm |
694nm |
|
Cy3™ /Cy5™ FRET |
550nm |
670nm |
|
Cy3™ |
552nm |
570nm |
|
Cy5.5™ |
675nm |
694nm |
|
Cy5™ |
649nm |
670nm |
|
Cy7™ |
743nm |
767nm |
|
Cycle 3 GFP |
395nm |
507nm |
|
Dansyl cad***erine |
335nm |
518nm |
|
Dansylchloride |
380nm |
475nm |
|
DAPI |
358nm |
461nm |
|
Dapoxyl® |
373nm |
574nm |
|
Dendra2 Green |
490nm |
507nm |
|
Dendra2 Red |
553nm |
573nm |
|
Di-4 ANEPPS |
488nm |
605nm |
|
Di-8 ANEPPS |
468nm |
635nm |
|
Di-8-ANEPPS non-ratiometric |
530nm |
620nm |
|
DiA |
456nm |
591nm |
|
DiA (4-Di-16-ASP) |
491nm |
613nm |
|
DiD (DilC18(5)) |
644nm |
665nm |
|
DIDS? |
341nm |
414nm |
|
DiI (DiIC18(3)) |
549nm |
565nm |
|
DiO (DiOC18(3)) |
484nm |
501nm |
|
DiR (DiIC18(7)) |
750nm |
779nm |
|
DM-NERF (4.5_6.5 pH) |
510nm |
536nm |
|
Draq5 |
647nm |
683nm |
|
Dronpa Green |
503nm |
518nm |
|
DsRed2 (Red Fluorescent Protein) |
558nm |
583nm |
|
DsRed-Express |
557nm |
579nm |
|
荧光染料 |
激发波长 |
发射波长 |
|
DsRed-Monomer |
556nm |
586nm |
|
DyLight 350 |
352nm |
435nm |
|
DyLight 405 |
399nm |
433nm |
|
DyLight 488 |
492nm |
517nm |
|
DyLight 549 |
554nm |
569nm |
|
DyLight 594 |
592nm |
616nm |
|
DyLight 633 |
623nm |
645nm |
|
DyLight 649 |
652nm |
667nm |
|
DyLight 680 |
677nm |
705nm |
|
DyLight 750 |
751nm |
772nm |
|
DyLight 800 |
770nm |
795nm |
|
EBFP |
380nm |
440nm |
|
EBFP2 |
385nm |
448nm |
|
ECFP |
433nm |
475nm |
|
EGFP |
488nm |
509nm |
|
EGFP/DsRed 2 FRET |
470nm |
585nm |
|
EGFP/Rhod-2 FRET |
488nm |
571nm |
|
ELF®-97 alcohol |
345nm |
530nm |
|
Emerald |
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